The current investigation was made to analyze the impact of different treatments on Arbuscular Mycorrhizal Fungi (AMF), Azospirillum (AZM), Market waste compost (MWC), Nitrogen (N), Phosphorus (P) and Potassium (K) are organic and inorganic fertilizers on several antioxidant enzyme activities like Catalase (CAT), Peroxidase (POD) and Polyphenoloxidase (PPO) of Arachis hypogaea L. To analysis of the enzyme activities were estimated at different day’s intervals. The results of this experiment revealed that combined of bio-fertilizers (T4) significantly increased antioxidant enzyme activities of the crop plant. In general, it might be concluded, further study on the log term of application of bio-fertilizers should be good tool to improve the crop productivity and mass and soil health.