The present study report is an approach to enshrine the rich ancestral bio cultural knowledge of Ahom (Sivasagar district) & Matak community (Dibrugarh district) of Assam (India) to treat dysmenorrhea. This study report encompasses total 17 types of medicines prepared from 25 plant species belonging to 18 families. It also clearly exhibits the procedure of preparation of medicine along with the prescribed doses. The plant parts used for the purpose are bark, stem, root, shoot apex, leaf, seed, Fruit, whole plant. On accordance with the distribution of habits, the no. of herbs, shrubs, tree, Climber are found to be 10,3,6,6 respectively.