Plants have been an important source of medicine with qualities for thousands of years. Phytochemicals are the chemicals extracted from plants. These organic chemicals are classified as primary or secondary constituents, depending on their role in plant metabolism. GC-MS method used for the analysis of the obtained extract can be an interesting tool for testing the amount of some active principles in herbs used in various industries. The aim of this study was to carry out for identification of bioactive compounds from the plant leaves ethanolic extract of Sansevieria roxburghiana by Gas chromatography and Mass spectroscopy (GC-MS). GCMS analysis of ethanolic extract was done by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of various compounds like Benzaldehyde, 2-nitro, Tetradecanal, 1-Octadecanol, Hexadecanoic acid, Hexadecane, 1-Heptadecanol, 2-Hexadecen-1-ol, 3, 7, 11, 15-tetramethyl-, [R-[R*, R*-(E)], 9, 12 Octadecadienoic acid, 9-Octadecenoic acid of Sansevieria roxburghiana leaves. These findings support the traditional use of Sansevieria roxburghiana leaves in various disorders.