Direct and indirect organogenesis of Gymnostachyum latifolium var. decurrens: An endangered plant
Shameer MC, Sreenivas VK
The study aimed to evaluate the efficiency of direct and indirect organogenesis for large-scale multiplication, propagation and conservation of Gymnostachyum latifolium var. decurrens, an endemic and endangered plant. Shoot tips and nodal explants were used for direct organogenesis. The highest frequency of shoot initiation from node and shoot tip was observed on MS medium with 2.22 M BAP. Node is used as a source of explant for callogenesis. High frequency of green and friable organogenic callus induction (95%) was observed from the node on MS medium supplied with 13.56 M 2,4-D. The regeneration frequency of shoot induction from the nodal derived calli was high (86%) when MS medium supplemented with 11.10 M BAP. Microshoots regenerated via direct as well as indirect organogenesis were rooted best in ½ MS medium containing 10.74 M NAA. The well rooted plantlets were successfully established in the soil.