Micropropagation of Buchanania lanzan spreng.-an endangered medicinal plant of the Western Ghats
Ajith S, Krishna V, Ravi Kumar S
Micropropagation protocol was developed for an endangered medicinal plant Buchanania lanzan (Family Anacardiaceae) via direct organogenesis from leaf explant. The chironji oil obtained from the seeds used in nutraceuticals and cosmetics and the leaf extract is used as an antioxidant and antidiarrheal drug. A method for rapid In vitro propagation of Buchanania lanzan (L.) was developed In vitro seeds culturing. The seeds sterilized thoroughly and inoculated M.S media. Supplemented with GA3 and BAP leaf explant obtained from seedling used for In vitro shoots and roots development. In vitro seed germination show a 79.33% response to concentration on MS+GA3 200mg/l. Direct organogenesis using the leaf explant treated with thidiazuron (TDZ), and Coconut water at 2 mg/l, 2 ml/l respectively were found to be optimum for direct shoot induction and growth with an average of 3.17 ± 0.37 shoots per explants. Successful In vitro rooting was induced from the cut end of the micro shoots. The maximum number of roots was obtained on 3.39 ± 0.22 on MS + NAA 1.5 mg/l. The regenerated shoots along a well-developed root have been effectively acclimatized then established within poly cups containing base rite afterward transferred to earthen pots containing garden soil and manure (2:1), then grown under greenhouse conditions with a 78 percent survival rate.