The work was an effort to study the impact of Phosphate solubilizing bacteria in biofertilizer production, which in turn enhances the nutrient quality of soil. The study presents the detailed account of isolation of phosphate solubilizing bacteria from the root nodule of Vicia faba from different locations of Thanjavur district, Tamilnadu state. Phosphorous plays an important role in plant metabolism as most of the soils are deficient in phosphorous. Phosphate solubilizing bacteria utilized as biofertilizer, which actually enhance the nutrient quality of the soil. Thirteen Rhizobium colonies separated and analyzed based on confirmation tests. biochemical studies. In some strains showed excellent zone of diameter. The effect of different concentration of glucose as a carbon sources on the Rhizobium strains with phosphate solubilization efficiency were calculated.