Vesicular-arbuscular mycorrhiza (VAM) symbiosis is a natural phenomenon of any plant community and play unique role in uptake of nutrient and water particularly in nutrient poor soil. Plants infected with VAM fungi can mobilize phosphorus and other nutrients which create a suitable environment for plant survive in nature. The agricultural soil of South West Bengal is low in organic carbon, nitrogen and phosphorus. Use of Vesicular-arbuscular mycorrhiza as bio-fertilizers creates favorable soil ecosystem where phosphorus, nitrogen, potassium and other nutrients get mobilized. In present agricultural practice excess chemical fertilizers, fungicides and pesticide are uses. The objective of the present investigation was to search out the easily available natural mycorrhizal inoculums present in the roots and rhizospheres of the luxuriantly growing weeds of Parthenium in this case all around. Plants inoculated with Parthenium rhizospheric mycorrhizal soil was proved more efficient inoculums, which could provide more growth compared to root inoculums, in the early age of seedling establishment. It was observed a positive aspect of Parthenium plants, whose roots and rhizospheric soil may be used as VAM inoculums for higher growth of Triticum aestivum L and Sesamum indicum L.