GC-MS analysis of phytochemical compounds in the ethanolic extract of flower of three species of Ipomoea
Leena Stephen, AK Bopaiah
The aim of this study was to phytochemically screen the plant metabolites present in the plant material(flower) in ethanolic extract of three species of Ipomoea namely Ipomoea bereviensis, Ipomoea cairica and Ipomoea hederacea, qualitatively using gas chromatography-mass spectroscopy (GC-MS). The shade dried flowers were extracted with ethanol. The three samples were filtered and derivatised using MoX reagent and BSTFA: TMCS. The analysis showed peaks with low and high molecular weight revealing the presence of 28 compounds in I. bereviensis, 36 compounds in I. cairica and 29 compounds in I. hederacea which were identified in comparison to the fragmentation patterns in the resulting mass spectra with those published in literature and using the National Institute of Standards and Technology (NIST), Mass Spectral Database of the gas chromatograph–mass spectrometer. Phytochemical screening affirmed the presence of mostly sugars, sugar alcohols, ethers and lipids. These results will consequently be discussed in the light of their putative biological or therapeutic relevance and if pursued further can lead to drug development. The outcome of this preliminary screening, which is a valuable step in the detection of bioactives will also help in discovering the bioactive profile of these particular species.