Comparative study on the extracellular amylase activity of amylolytic bacteria isolated from different soil types and earthworms gut
Cecilia Mary, Eve Anchal Vaj, Arya Subash, Nivya Mariam Paul
An analysis for the abundance and diversity of amylolytic bacteria of earthworm gut and nine different soil types viz. food waste disposing soil, municipal waste disposing soil, kitchen waste disposing soil, soil from three different wetlands and mangroves, flood affected and unaffected paddy field soil and goat excreta disposing soil was carried out. A total of 52 bacterial isolates (25 from soil and 27 from earthworm gut) were primarily selected for their amylolytic potential. Among them, the percentage of amylolytic bacteria was higher in soil (16) than earthworm gut flora (11). Among soil bacterial flora, the percentage of amylolytic bacteria was higher in waste disposing soil (7) and lower in bacteria from agricultural field (4). Among earthworm gut flora, the percentage of amylolytic bacteria was higher in earthworms isolated from wetlands mangroves (5) and waste disposing area (4) and least in earthworms isolated from agricultural field (4). The highest activity among soil micro biota was shown by Es02s1, 20mm, (flood unaffected soil) followed by Ac01s3, 19.6mm, (kitchen and farming waste dumping soil). The highest activity among earthworm gut microbiota was shown by Cm03m, 18mm (bacteria isolated from the mid gut of earthworm). As the importance of amylase production was increasing in various industrial sectors, it is significant to find more and more cheaper and easily available sources for it. The current work is a small attempt on finding out a better, cheaper and easily available source for the amylolytic bacteria between different soil types and gut flora of earthworms.