The effects of different plant growth hormones on shoot proliferation and root development during induction, multiplication and rooting stagewas studied for meristem culture of banana cv. Gaja Bantala. MS medium supplied with 6 mg/l BAP in addition to 2 mg/l IAA and 100 mg/l ADS showed remarkable results for initial culture. During multiplication culture the explants cultured on MS medium along with 3 mg/l BAP + 1 mg/l IAA + 0.25 mg/l NAA developed highest number of shoot buds. For root induction MS medium with Activated charcoal (50 mg/l) and 1 mg/l NAA gave most suitable result.