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VOL. 6, ISSUE 2 (2021)
Antibacterial activity of Asparagus racemosus willd. and Ocimum gratissimum L. in urinary tract infection from in and outdoor patients of Duta Deepti Satsang Charitable Hospital, Deoghar, Jharkhand, India
Authors
Ratan Dhar, PK Baruah
Abstract
Asparagus racemosus Willd. (Asparagaceae) and Ocimum gratissimum (Lamiaceae) are commonly used traditional medicinal plants in Jharkhand for urinary tract infections (UTI). In the present investigation, an attempt was made to study the antimicrobial activity of A. racemosus and O. gratissimum against three predominant UTI bacteria obtained from in and outdoor patients of Duta Deepti Satsang Charitable Hospital, Deoghar, Jharkhand, India. The antibacterial activity of plant extracts was screened by the disc diffusion method of Kirby-Bauer sensitivity test against three predominantly occurred UTI bacteria viz., E. coli (DDSCH54), Klebsiella pnemonae (DDSCH62) and Proteus mirabilis (DDSCH72). Among all the extracts, the root ethanol extract of A. racemosus was found to exhibit better antimicrobial activity against E. coli (DDSCH54) and K. pnemonae (DDSCH62) at 20 mg/ml concentration whereas in case of O. gratissimum, the seed methanol extract showed more antimicrobial activities against all the three UTI bacterial isolates as compared to control (nitrofurantoin). Root ethanol extract of A. racemosus exhibited maximum inhibition 9.33±0.88 mm against E. coli (DDSCH54) and 9.33±0.66 mm against K. pnemonae (DDSCH62). However, the seed methanol extract of O. gratissimum inhibition 9.33±0.22 mm against E. coli (DDSCH54); 9.4±0.3 mm and 9.4±0.6 mm against K. pnemonae (DDSCH62) and P. mirabilis (DDSCH72) respectively. The minimum inhibitory concentration (MIC) revealed no inhibitory effect at 1.25 and 2.5 mg/ml concentration of both the ethanol extract against test organisms. The ethanol root extract of A. racemosus showed inhibition at 0.156 mg/mL and seed methanol extract of O. gratissimum exhibited inhibition zones at 0.078 mg/mL as compared with ethanol extract. From overall MIC’s observation, methanol extract of O. gratissimum at 20.0 mg/ml concentrations showed maximum zone of inhibition for all the test organisms. This investigation proved the efficacy of both the traditional medicinal plants for antibacterial activity against predominate UTI bacteria.
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Pages:547-553
How to cite this article:
Ratan Dhar, PK Baruah "Antibacterial activity of <em>Asparagus racemosus </em>willd. and <em>Ocimum gratissimum</em> L. in urinary tract infection from in and outdoor patients of Duta Deepti Satsang Charitable Hospital, Deoghar, Jharkhand, India ". International Journal of Botany Studies, Vol 6, Issue 2, 2021, Pages 547-553
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