Endophytic fungi are defined as fungi that grow in healthy plants tissues without causing any signs of disease or damage to the host plants. In the present study we isolated endophytic fungi from three different spice plants such as Clove (Syzygium aromaticum), Cinnamon (Cinnamomum verum) and Pepper (Piper nigrum) and cultivated in potato dextrose broth. The extracellular metabolites were extracted using ethyl acetate solvent. Further we evaluated the antimicrobial activity of the ethyl acetate extract using Kirby-Bauer disc diffusion technique. In total 18 endophytic fungi were isolated from the spice plants and identified morphologically as Fusarium spp. and Colletotrichum spp. The thin layer chromatography of the ethyl acetate extract showed 3 prominent bands having a Rf value of 0.31, 0.43 and 0.52, respectively. In conclusion, the Fusarium sp. isolated from clove plant showed significant antibacterial activity against Klebsiella sp. and Citrobacter sp.