Carotenoid (lycopene) research is growing significantly, as several epidemiological and clinical trials demonstrate consumption of tomato-rich diet reduces the risk of cardiovascular diseases and prostate cancer. The aim of the study was to isolate and analyse high pure lycopene from ripened tomatoes to target enhanced lycopene bioavailability through nano-delivery using phytoconstituents like zein corn protein and alginate. Standardized procedure yield high pure (93.3%) lycopene and developed UPLC/MS method for lycopene analysis is rapid and simple. As free lycopene is unstable, we synthesised lycopene encapsulated zein-alginate nanoparticles (LYZA) and characterized its physicochemical properties. LYZA nanoparticle produced a cumulative 195 ± 31 nm particle size, stable zeta potential (-30.4 ± 3 mV), PDI (0.09 ±0.02), and amorphous in nature. Further, the release kinetics exhibited inhibition of lycopene release at acidic conditions and sustained controlled release at intestinal pH. Lycopene bioaccessibility of LYZA was significantly higher than digestion of synthetic lycopene (LYMM) and tomato (TMM) supplements. The results demonstrate high stable and sustainable lycopene supplements as a promising application in nutraceuticals/pharmaceuticals.