Antibacterial and GC-MS analysis of stem and leaf of Premna paucinervis (C.B. Clarke) gamble (Lamiaceae)- An endemic and rediscoverded species
Steffy Francis, V Anand Gideon, S John Britto
The present study aims to bring out the bioactive compounds of Premna paucinervis and evaluate the antibacterial activities of its crude extracts. The antibacterial activity was determined in the extracts using zone of inhibition method and were tested against B. cereus, B. subtilis, E.coli, E.aerogenes, K.pneumoniae, P.mirabilis, P.vulgaris, P.aeruginosa, S.paratyphi, S.marcescens, S.aureus, S.pneumoniae and V.cholerae. Zone of inhibition of the extracts were compared with that of control for antimicrobial activity. The results of the leaf extract showed notable inhibition of the bacterial growth against the tested organisms. The microbial activity of the P. paucinervis might be due to the presence of various secondary metabolites. Hence, this plant can be used to identify the specific bioactive compounds which may serve as leads in the development of new antimicrobial agents. Gas Chromatography- mass spectrometry (GC- MS) analysis of stem and leaf extracts showed presence of 45 and 65 compounds respectively. N-Hexadecanoic acid, Oleic Acid, Stigmasta-5, 20(22)-dien-3-ol and 9, 12-Octadecadienoic acid (Z, Z) - were the major bioactive components found in the stem extract. 2-Propenoic acid, 3-(4-hydroxy-3-methoxyphenyl)-, methyl ester, 2-Methoxy-4-vinylphenol, 6-Hydroxy-4, 4, 7a-trimethyl-5, 6, 7, 7a-tetrahydrobenzofuran-2(4H)-one and Benzofuran, 2, 3-dihydro- were the major components found in the leaf extract.