Phytomediated synthesis and characterization of silver nanoparticles from the leaf extracts of Clausena anisata (Willd.) Hook. F. Ex Benth. and its antimicrobial activity
M Thilagam, B Esakkiammal, P Mehalingam
Development of biologically inspired phytomediated synthesis of silver nanoparticles is evolving into an important branch of nanobiotechnology. In the present investigation, we report the phytomediated synthesis of silver nanoparticles (AgNPs) employing the leaf extract of Clausena anisata (Willd.) Hook.f. ex Benth (Rutaceae). The synthesized Ag-NPs were characterized by UV-visible, X-ray diffraction (XRD), Fourier-transform infrared (FT-IR), Scanning electron microscopy (SEM), and Energy dispersive X-ray (EDX). Formation of silver nanoparticles was confirmed by the change of colour from pale yellow to dark brown in colour. These results authenticated that the appearance of AgNPs was analyzed by UV- visible spectrum around the peak 420 nm. XRD (X-ray diffractometer) demonstrated the formation of crystalline AgNPs with FCC structure having an average crystalline size of 20.42 nm from XRD profile. FT-IR analysis revealed the presence of different functional groups in the synthesized AgNPs. Antimicrobial activity of the synthesized silver nanoparticles was evaluated against Gram positive and Gram negative bacteria such as Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and fungus Candida albicans. Both the leaf extract and synthesized silver nanoparticles from the leaves of Clausena anisata showed moderate antimicrobial activity.