in vitro cytotoxicity of Dioscorea oppositifolia L. against breast cancer cell line MCF-7
Uma Maheswari Rajadurai, Abirami Hariharan
Green synthesized silver nanoparticles from Diosgenin, an active principle of Dioscorea is a steroidal saponin has been characterized by UV-Vis spectroscopy, SEM, TEM, DLS and FTIR. Diosgenin act as a reducing agent converts silver ions to AgNPs in a rapid and eco-friendly manner. The UV-Vis spectra of Diosgenin samples depicts surface plasmon resonance peak at 410-431 nm. TEM image of the sample shows well-dispersed silver nanoparticles with an average particle size of 13 nm. The three dimentional structure of silver nanoparticles was seen through SEM analysis. Diosgenin mediated silver nanoparticles was found to be rod shaped and DLS studies showed approximate size of nanoparticles and their zeta potential value was proved by their high stability nature. FTIR showed functional groups and the stretch of bonds which responds to nanoparticles synthesis were elucidated. Breast cancer is the topmost cancer in women both in the developed and the developing world. The incidence of breast cancer is increasing in the developing world Progress. According to WHO Report, issued in August 2012, provides an update with the latest data on breast cancer incidence and mortality. Adequate therapy is needed for breast cancer that, although not amenable to early detection and treatment, Nanotechnology has high potential for breast cancer treatment. In vitro cytotoxicity activity of Diosgenin and its nanoparticles against most prevailing dangerous life threatening disease in women, breast cancer using MCF-7 Cell line. Diosgenin has shown 49% inhibition against breast cancer cell line whereas diosgenin mediated silver nanoparticles has shown 100% inhibition against MCF-7 breast cancer line. This is the first report of synthesizing silver nanoparticles from Diosgenin, Dioscorea oppositifolia plant active principle which is a triterpenoids steroidal saponin and are used as cortico-steroidal drug and its efficacy was successfully analysed for breast cancer treatment.