Given the astonishing number of microbial organisms known to exist, measuring microbial diversity is one of the most difficult tasks in contemporary microbiology. Endophytes make up a significant part of microbial diversity. In the present research, the Santalum album, a significant plant with major ethnobotanical uses, was analyzed for the presence of endophytic fungi from the Bisle region, Western Ghats, Karnataka, India, during all three seasons of 2017. Normal isolation procedures were used to separate fungal endophytes from healthy plant sections, including leaves, roots, and petioles. Fungi were isolated and grouped based on colony morphology and identified based on mycelia shape and structure, sexual and asexual Reproductive characters, attachment of spores, and cultural conditions. A total of 54 fungal isolates were obtained from 4 fragments. Leaf fragments had the highest isolation frequency (0.65) during winter, and summer's petiole fragments had the least isolation frequency (0.15). Cochliobolus sp.and Curvularia sp. commonly appeared in all seasons. Among all endophytic fungi classes, Deuteromycetes were leading over other fungal classes. Endophytic fungi were found to have a rich diversity of Shannon-Weiner and Simpson's indices. These indices point to an even and stable distribution of different fungal isolates. The findings contribute to our knowledge of the diversity of endophytic fungi present in host plant tissues.