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VOL. 6, ISSUE 2 (2021)
Trigonella foenum-graecum sprouted seed aqueous extract exhibits strong anticoagulant and fibrinogenolytic activities: Role of serine protease
Authors
Thoyajakshi RS and Poornima D
Abstract
Trigonella foenum-graecum sprouted seed aqueous extract (TFS extract) exhibited fibrinogenolytic, anti-coagulant (both in vitro and in vivo) and anti-platelet aggregating activities. TFS extract hydrolysed casein with a specific activity of 0.6 U. TFS showed increased activity in terms of clotting time of citrated plasma by 2.04 and 1.61 folds in RT and PT respectively. TFS extract inhibited ADP induced platelet aggregation to an extent of 65%. Further, the in vivo studies showed the prolonged tail bleeding time in dose dependent study using mouse model, which supported anticoagulant property. The TFS specifically prolonged the clotting time in PT (from 19±1 sec to 30±0.58 sec) and RT (from 3.6±0.3 min to 7.44±0.09 min) revealing the involvement of extract in extrinsic pathway of coagulation. TFS extract completely hydrolyzed all the chains Aα, Bβ and γ chains of fibrinogen. The proteolytic activity of TFS extract was completely inhibited by PMSF, however E- 64, EDTA and pepstatin-A did not, indicating the presence of serine type of protease. TFS extract was devoid of hemolytic, edema inducing and hemorrhagic activities suggesting its non-toxic nature. TFS has significant anticoagulant bioactive molecule/s which can be useful for further thrombotic disorder treatment.
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Pages:620-627
How to cite this article:
Thoyajakshi RS and Poornima D "<em>Trigonella foenum-graecum</em> sprouted seed aqueous extract exhibits strong anticoagulant and fibrinogenolytic activities: Role of serine protease ". International Journal of Botany Studies, Vol 6, Issue 2, 2021, Pages 620-627
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