Isolation of cement degrading filamentous fungi and screening of their efficacy for biocementation
N Uma Maheswari, P Priyanga
Cement dust is usually grey powder before mixed with other materials and water. It is one of the major source of environmental pollution. The purpose of the present study highlighted that degradation ability of filamentous fungi and efficacy of biocementation. Soil samples was collected from cement dumping sites in around Tiruvarur District, Tamilnadu, India. Soil samples are subjected to serial dilution and identification by Lactophenol Cotton blue mounting technique and standard manuals. Identified filamentous species are Aspergillus niger and Fusarium species. Then it was allowed to grown in different cement concentration (1g, 2g, 3g, 4g, 5g) using silicate basal medium with optimized parameters such as pH, temperature, and inoculum concentration. Gravimetric estimation for weight reduction also preferred. Compared to Fusarium species urease positive Aspergillus niger was able to produce gluconic acid at pH 7 and Biocementation production was also noticed. Gluconic acid production was characterized by paper chromatography (colorspot) and HPLC.