Ammi majus L. belongs to family Apiaceae is one of the unique source of various types of chemical compounds, which are responsible for a variety of activities. It has various medicinal properties and is useful in the treatment of leucoderma, vitiligo, as a diuretic, emmenogogue, abortifacient and blood purifier. A. majus extracts showed significant biological activity such as antiviral, antimicrobial, antioxidant, relaxant effect, cardiovascular effect, hypotensive etc. More than fifty bioactive phytochemicals were isolated from the particular plant species but the main groups of phytochemicals that are potential against different biological activities are flavonoids and coumarins. The purpose of this review was to provide a brief summary of the knowledge of biopharmaceutical effects of secondary metabolities of Ammi majus L. and the mechanism of their action. As the worldwide scenario is now changing towards the use of non-toxic plant products having traditional medicinal use, development of modern drugs from Ammi majus L. should be emphasized for the control of various diseases.