In recent years green synthesis of silver Nanoparticles has been increased for diverse application. In this study we proposed lamiaceae family plant. Pogostemon benghalensis for synthesis of silver nanoparticles and their efficacy in antibacterial activity against five humen pathogens. The direct method was chosen for silver nanoparticles synthesis using water extract of Pogostemon Benghalensis. Green synthesized silver nanoparticles were characterized by UV-Vis spectroscopy, FTIR, SEM, EDS. The UV-Vis spectro depicts surface Plasmon resonance peak present at 450nm. The three dimensional structure of silver nanoparticles was seen through SEM analysis. The observed FT-IR peaks showed functions groups and the stretch of bonds that responds to Agnps synthesis. The synthesized nanoparticles showed remarkable antibacterial activity against three gram positive bacteria strains Staphylococcus aureus, Enterococcus faecalis, Enterococcus phyogenes and two gram negative bacterial Strains Salmonella typhi, Enterobacter aerogenes. The highest antimicrobial activity against Enterococcus pyogenes (16mm) and the lowest antibacterial against Enterococcus faecalis (7mm). The present suggest that Agnps can be used for potential of antibiotic drugs.