In an attempt to record the ethnomedicinal plants utilized by the Kani tribal settlement of the Pechiparai forest range (southern Western Ghats) in Kanyakumari district of Tamil Nadu, India, an ethnobotanical survey was carried out. The required data was put together after a direct interview of the local Kani traditional practitioners. It was deduced from the current examination that wild resources served as the major source plants in the traditional systems. A total of 59 species of medicinal plants belonging to 38 families were documented that are utilized for the treatment of skeleto-muscular system ailments such as rheumatism, headache, fatigue, bone dislocations, body heat, joints aches with inflammations and nervous conditions. The Kani tribal people’s practical first-hand knowledge about the local plants and its utility is a significant practice taking into consideration the deteriorating conventional systems and traditions. Similar efforts ought to be taken to verify and assess the viability of these medicinal herbs utilized by the tribal groups.