ARCHIVES
VOL. 6, ISSUE 3 (2021)
Quantitative estimation of quercetin in Nelumbo nucifera leaves by HPTLC
Authors
Haritha Chowdary Darapaneni, Prachet Pinnamaneni, Rama Rao Nadendla
Abstract
A sensitive and reliable HPTLC method was developed for the quantitative estimation of Quercetin in Nelumbo nucifera leaves. Chromatographic analysis was performed by n-Butanol: Glacial Acetic Acid:Water:0.1% Formic Acid (7:1:1:0.25v/v/v/v) as mobile phase, TLC Silica gel 60 F254 as stationary phase with a dosage speed of 20µL/sec and detection was carried out at 254nm. Rf value of Quercetin was found to be 0.907. The developed method was validated for system suitability, linearity, accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ) and robustness according to ICH guidelines. Linearity of Quercetin was 2000-10000ng/spot with a correlation coefficient of 0.999. The % recovery ranged between 98-102% and % RSD was <2. Hence, the proposed method can be applied for routine analysis.
Download
Pages:464-469
How to cite this article:
Haritha Chowdary Darapaneni, Prachet Pinnamaneni, Rama Rao Nadendla "Quantitative estimation of quercetin in <em>Nelumbo nucifera </em>leaves by HPTLC ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 464-469
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.