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VOL. 6, ISSUE 3 (2021)
Evalution of total phenolic and flavonoid content, free radical scavenging activity and phytochemical screening of fruits of Raphanus caudatus l.
Authors
Vaishakhi Patel, Mayur Prajapati, Nainesh Modi
Abstract
This study aimed to evaluate the phytochemical analysis and the total phenol and flavonoid content and antioxidant activity of fruit extract of Raphanus caudatus L. from brassicaceae family. The fruits of Raphanus caudatus L. were dried and extracted with two different solvents (methanol and chloroform). Secondary metabolites are important for medicinal activity of plants. So in the present study phytochemical screening of fruits of Raphanus caudatus L. was carried out. Quantitative analysis of phenols was carried out using folin- ciocalteu method. Gallic acid was used as the standard for assessment of phenols. Evaluation of total flavonoid content was carried out by using quercetin as a standard. The antioxidant activity of extract was determined by DPPH assay. Preliminary analysis of each crude extract shows the presence of alkaloids, carbohydrates, glycosides, phenols, flavonoids, terpenoids, steroids, protein. The crude methanol extract was collected of a remarkable quantity of TPC and TFC 186.66±1.013 mg/GAE/g and 26.83±1.166 mg/QE/g of sample respectively. The IC50 value of crude methanolic extract was 612.58±1.41 µg/ml.
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Pages:499-504
How to cite this article:
Vaishakhi Patel, Mayur Prajapati, Nainesh Modi "Evalution of total phenolic and flavonoid content, free radical scavenging activity and phytochemical screening of fruits of <em>Raphanus caudatus </em>l. ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 499-504
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