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International Journal of
Botany Studies
ARCHIVES
VOL. 6, ISSUE 3 (2021)
Isolation and characterization of endophytic microbes from selected medicinal plants and its antimicrobial activity
Authors
N Uma Maheswari, P Kokila
Abstract
Endophytes are ubiquitous and have been found in all the species of plants and their tissues such as stem, leaves, roots and petioles etc. Endophytes may benefit host plants by preventing pathogenic organisms from colonizing them. Now a day, studies on endophytes of medicinal plants had been given due to attention as they tend to produce natural products beneficial for a human being. Hence the present study was planned to isolate endophytic bacteria and fungi from fresh leaves of medicinal plants, collected from Herbal Garden, STET Women’s college, Mannargudi, Thiruvarur (Dt) namely Ocimum tewurflorum L, Cardiospermum halicababum L, Acalypha indica L, Mukia maderaspatana L, Eclipta prostrate L, Phyla noditlora L, Solanum nigrum L, Andrographis paniculata L, Boerhavia diffusa L, Centella Asiatica L, Lawsonia inernis L, Solanum Trilobatum L, Clitoria ternatea L, Phyllanthus nuiri L, Mentha spiata L, Justicia adhatoda L, Vitex negunda L, Murraya koenigi L, Coriandum Sativum L, Catharanthus roseus L.,screening of phytochemical consitutuents,and antimicrobial activity was also done. Although Totally 20 medicinal plants were selected,bacterial species isolated from medicinal plants and it was identified as Bacillus subtilis, Streptococcus pyogenes, E. coli, Staphylococcus aureus, Staphylococcus saprophiticus, Micrococcus by Gram staining and biochemical characteristics. Phytochemical constituents such as steroids, terponoids, glycosides, flavonoids and saponins were present in some of these medicinal plants. The isolates were also screened for extracellular biosynthesis of Silver nanoparticles for which UV-VIS absorption was recorded. Among the isolates A. Niger and E. coli was present in all medicinal plants.
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Pages:521-526
How to cite this article:
N Uma Maheswari, P Kokila "Isolation and characterization of endophytic microbes from selected medicinal plants and its antimicrobial activity ". International Journal of Botany Studies, Vol 6, Issue 3, 2021, Pages 521-526
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