The present investigation deals with Antimicrobial analysis on Floribundariawalkari a moss plant. The whole part of the plants were screened for antimicrobial evaluation. The antimicrobial activities tested using various extracts viz.. Ethyl acetate, chloroform, ethanol and aqueous extracts against nine bacterial and seven fungal strains. The antibacterial studies revealed that the most susceptible microorganisms to Escherichia coli and Pseudomonas aeruginosa and fungal activity the ethonolic extracts showed good antifungal activities against the test ed fungi. Floribundaria wlkari could be exploited in the Infectious management of various bacterial and fungal diseases.