Traditional practice for curing diseases using locally available medicinal plants is a culture adopted by Indians from prehistoric period. Cucurbitaceae members are also used as medicine for various sickness, one among them is Zanonia indica L. which is selected for the study. The aim of the investigation was to extract the phytochemicals and to examine the anti-oxidant property of the selected plant. Powder of dried fruit of Z. indica was subjected to Soxhlet extraction using different solvents to obtain crude extracts. Hexane, chloroform, acetone, methanol and distilled water was used for extraction. Crude extracts of these solvents showed the presence of phenols, alkaloids, terpenoids, flavonoids, steroids, carbohydrates, tannins, saponins, glycosides quinones, resins, and proteins in preliminary analysis. Quantitative analysis of crude extracts using HPLC showed that the acetone and methanol extract was rich in total alkaloids, total phenols, total flavonoids and total terpenes. Antioxidant activity of fruit extracts of Zanonia indica using DPPH assay, ABTS radical scavenging assay and FRAP assay revealed the presence of antioxidant property. Methanol and acetone extract of fruit was rich in antioxidants. This study evidenced the presence of bioactive compounds as well as its antioxidant properties and hence proves the traditional practice of the selected plant was appropriate.