The current research focus on the study of moss diversity and secondary metabolite analyses using FTIR and further research was undertaken on these bryophytes that were preserved by ex-situ method in the lab. Mosses collected from Baramati and Revadanda Borli ranging from wet shady to temperate to tropical climates. These five bryophytes species as Fissidens crenulatus, Steeriophyllum anceps, Hyophila involuta, Riccia discolor and Targionia hyophylla maintained by Ex-situ preservation and analysed. Riccia discolor > Targionia hyophylla > Hyophila involuta > Steeriophylum anceps > Fissiden secrenulatus are examples of secondary metabolites that demonstrate broad tendencies of decreasing halo compound concentration.