The current study focus on fungal isolation of mosses in the Baramati of Maharashtra's District Pune. The serial dilution method and Potato Dextrose Agar (PDA) media were used to isolate soil fungus. Fungi were identified based on their morphological characteristics. Throughout the study, a total of 15 fungal species were identified. The contribution of Deuteromycotina was 53.33% followed by Zygomycotina 26.66%, Ascomycotina 13.33% and Mastigomycotina 6.66 %. During the study., Rhizopus oryzae, Rhizopus Stolonifer, Rhizopus sp., Mucor mucedu, Trichoderma atroviride, Trichoderma viride, Trichoderma harzianum, Aspergillus sp., Aspergillus brunneoviolaceus, Aspergillus ochraceus, Aspergillus niger, Aspergillus fumigatus, and Penicillium aurantiogriseum. The predominant genera Pythium sp. and Helminthosporium sp. were detected less often. The FTIR investigation having presence high concentration of halo compounds, which are resistant to herbicides and insecticides. Other compounds that are present include high amount of vinyl ether, amine, aromatic amine, phenol, primary amide, alkene, sulfonate, fluro compound, alkyne, and aromatic ester, etc.