ARCHIVES
VOL. 6, ISSUE 5 (2021)
Nephroprotective activity of alkaloidal fraction of Alstonia scholaris
Authors
Ashok Kumar Kushwah, Neetesh Kumar Jain
Abstract
Aim: This study specifically investigated the possible protective effects of alkaloid fraction of selected plant against oxidative stress and renal impairment induced by suitable agents. Experimental Work: Around 500 gm dried leaves of Alstonia scholaris were coarsely powdered weighed and filled in Soxhlet apparatus for extraction. First the powdered drug was defatted with petroleum ether (60°C-80°C); Defatted drug was then dried and again filled in soxhlet apparatus for successively extraction with methanol and water as solvent. The ethyl acetate soluble fractions were dried and kept in air tight container and it is named as ALKALOID FRACTION (AF) of leaf extract. Cisplatin is the highly effective chemotherapeutic agent widely used in the treatment of a variety of cancers. However, its clinical utility is limited due to its most common adverse effect i.e. nephrotoxicity in many patients. For this method, the samples of the blood was withdrawn, collected in absence of sodium citrate and allowed to clot. The clotted blood was centrifuged for 10 minutes at 2500 rpm. The obtained clean and clear serum was refrigerated at 200C for the analysis of blood urea, serum creatinine (CR), and uric acid (UA) level by adopting & utilizing colorimetric assay kits as per given procedure. Results: There was clear variation and alteration (increase) in the levels uric acid, creatinine and urea levels when compared to normal group animals. 50 and 100mg/kg dose of alkaloidal fraction was able to bring down the elevated values of uric acid, creatinine and urea levels towards normal, when compared to toxic control groups. Alkaloidal fraction showed a most highly significant effect (P>0.001) as compared to other disease control. Conclusion: The results obtained in this study have shown that alkaloidal fraction displayed significant nephroprotective activity in acute and chronic conditions. Besides from the obvious therapeutic importance, these components would be useful in understanding the mechanism of diseases with higher levels of cellular and molecular level.
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Pages:101-104
How to cite this article:
Ashok Kumar Kushwah, Neetesh Kumar Jain "Nephroprotective activity of alkaloidal fraction of <em>Alstonia scholaris</em> ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 101-104
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