Aim: Effect of Syringic Acid on Lysosomal and Anti-oxidant Enzymes in Arthritic Animals. Material and Methods: The acute oral toxicity studies were carried out according to the guidelines set by the Organization for Economic Co-operation and Development (OECD), revised draft guideline 423. The Wistar albino rats were divided into 5 groups of six animals in each. For the induction of chronic inflammatory response, FCA (0.1 ml) was injected through intra-articular injection in left ankle joint of rats on 0 day. Pre-induction baseline was taken prior to the injection of Freund’s Complete Adjuvant (FCA) measured by left paw volume of each animal at 0 day for the induction of arthritis in Wistar rats. The treatments with Syringic acid were given once daily from day of injection to 21st day. The severity of adjuvant arthritis was quantified by measuring the volume of the hind paw using Plethysmograph. Body weight was measured of all groups at zero days before immunization and at 21st day after treatments over by using a single pan weighing balance. Results: No toxic effects were observed at a higher dose of 500 mg/kg body weight of Wistar rats. Hence, 1/ 10th dose was selected as effective dose or therapeutic dose. The cut off value of 50 and 1/5 dose double of 100 mg/kg were selected for anti-arthritic and anti-inflammatory activity. The assessment made on the 21st day showed that the Syringic acid treatments at both doses (low and high) had moderately significant and highly significant effect and reduced (p< 0.01& p<0.001) the adjuvant-induced lesions in the respective treatment groups as compared with the arthritis control group. Syringic acid had moderately and highly significant increase in body weight (p<0.01 & p<0.001) as compared to arthritic rats. Administration of Syringic acid at dose of 100 mg/kg caused highly significant decrease (p<0.001) in MDA levels and increase in GSH and SOD activities. Conclusion: In conclusion, it can be stated that the Syringic acid have beneficial effects in long lasting reduction in rat paw edema, arthritic index and various lysosomal enzymes. It also showed a protective effect on arthritic rat joints without any toxic effect.