Pentatropis Plantlets were produced in vitro from the shoot and a nodal segment with an effective procedure developed with higher frequency with rapid rejuvenation. In vitro culture tubes were initiated by meristematic explants on MS medium with various growth hormones individually as well as combinations. Among the two concentrations used Benzyl amino purine and Kinetin with 2, 4-D the best for callus induction is 95% at 2.0 + 1.5 mg/l concentration. This clearly shows combinations of both used in cytokinins Benzyl amino purine alone at 3 mg/l induced maximum number of shoot buds when compared with KIN alone. Well-developed shoots were excised from cultured tubes and cultured on MS media having different concentrations like 0.1 to 1.2 mg/l and NAA is more effecient than IBA at 0.6 mg/l. NAA is the best concentration for auxin by proper rooting where 85% shoots were able to rooted within six weeks.