Hyperlipidemia is an abnormal increase of fats (lipids) in the blood and it can cause cardiovascular disease, diabetes and stroke worldwide. Hyperlipidemia induced by the oleic acid, increased the level of triacylglycerol and sterol esters. Atorvastatin is commercialy available drug, used to cure hyperlipidemia which cause side effect like diarrhea, stomach upset, muscle and joint pain. Rutin a flavonoid found in some fruits and vegetables have biological activity including antioxidant, anti-inflammatory, anti-diabetes, hepatoprotective, neuroprotective and nephroprotective. Hence, we have used rutin for hyperlipidemia treatment in a model system Saccharomyces cerevisae. Rutin does not affect cell growth at 0.5 mm, 1 mm concentrations. These results conclude that rutin can be used to reduce hyperlipidemia in Saccharomyces cerevisiae.