Rutin a flavonoid and natural compound found in certain plants, fruits, vegetables, buckwheat, Japanese pagoda tree, and Eucalyptus. Rutin is known to have biological activity antioxidant, nephroprotective, anti-inflammatory, neuroprotective, anti-diabetes and hepatoprotective. Atorvastatin, a commercial drug is used for treatment of hyperlipidemia which leads to some side effect in human like myopathy, weakness, myalgia, headache, dyspepsia, arthralgia and liver injury. In the present study we have evaluated rutin in reducing the hyperlipidemia genes TAG (LRO1 and DGA1) and SE (ARE1 and ARE2). These results suggest, the rutin can act as anti-hyperlipidemia effect in Saccharomyces cerevisiae.