The present study was aimed to determine the antibacterial activity of different extracts of Annona muricata. Dried seed powder was used for the extraction of different solvents such as chloroform, ethyl acetate, acetone, ethanol and distilled water. The solvent extracts were assessed for the antibacterial activity against Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhi by using agar disc diffusion method. Among the selected solvents, ethanolic extracts of Annona muricata was more active against bacterial pathogens than aqueous extracts. Staphylococcus aureus and Bacillus subtilis was highly sensitive to all the solvents. Least activity was noticed against Enterococcus faecalis.