Survey and collection of forty-three wood-decaying fungi were done from different localities of Paithan Tehsil, Aurangabad district (M.S.) India. From that seventeen genus and eighteen species were identified according to macroscopic and microscopic characters. The study of specimens was carried out with respect to botanical name, host, fruiting bodies dimension, spore dimension, altitude, latitude, and longitude. Based on observations Daldinia concentrica, Flavodon flavus, Ganoderma lucidum, Macrocybe pachymeres, Phellinus badius, and Schizophyllum commune are dominating macrofungi and Coriolopsis gallica, Earliella scabrosa, Gymnopilus purpureosquamulosus, Hypoxylon haematostroma, Inonotus rickii, Phellinus gilvus, Phylloporia pectinata, Pleurotus djamor, Porostereum spadiceum, Scytinostroma duriusculum, Tomophagus colossus, and Volvariella diplasiaare are rarely observed.