Introduction: Obesity is a disorder with excessive body fat that increases the risk of health problems. There is a vast prevalence of obesity in both developed as well as developing countries, and now it is affecting children as well. The World Health Organization reported that more than 1 billion adults are overweight, and at least 300 million of them are obese. India hits by the number of more than 135 million. Obesity is associated with other serious complications, making its management of utmost importance. However, current management with allopathy and surgeries are associated with various adverse effects. Hence, we conducted this study for evaluating the safety and efficacy of herbal drugs in managing obesity. Method: In the present study, an herbal formulation was evaluated for anti-obesity activity in Wistar rats (n=36). The anti-obesity potential of this formulation was studied on a high-fat diet (HFD)-induced obesity in male Wistar rats. In this study, chronic administration of HFD in rats produced hypercholesterolemia which led to an increase in the body weight, total cholesterol, triglycerides, and attenuation in the levels of HDL as well as changes in body temperature of animals. Then our formulation was tested for its efficacy to bring these values to normal. Results: Our formulation was able to bring down these values towards normal range which was comparable with marketed formulations. Conclusion: Hence, we can conclude that our polyherbal mixture possesses anti-obesity activity in preclinical study.