Mushroom is a macro fungus, belonging to the kingdom Mycota. Mushrooms are unique, because, they are neither animal nor plant. Mushrooms are heterotrophic organisms, they show a great diversity in morphology and preference for habitats. The present study aims to estimate the nutritional profiling of Pisolithus arrhizus (Scop.) Rauschert, (1959) a non edible wild mushrooms collected from Western Ghats of Karnataka, India. Sporomas of collected mushroom were analyzed for their proximate chemical composition (moisture, proteins, carbohydrates, fat, fiber and ash) major and minor mineral elements (N, P, K, Ca, Fe, Mg, Mn, Cu, Zn) and heavy metal contents (Pb, Cd, Cr, Ni) on dry weight basis. The result reveals that, Pisolithus arrhizus is rich in carbohydrates 47.87%, and low amount of fat 0.41% also contains moderate amount of protein 5.17%, fibre 13.14% followed by ash 17.92% and moisture 15.49%. The energetic contribution was found to be (215.85Kcal/100 g). Nitrogen (N) and potassium (K) are the most abundant minerals in Pisolithus arrhizus and showed higher concentration of N (3.19%) and K (1.56%), whereas Ca (0.10%) and Mg (0.08%) and studies mushroom were also assessed for their trace elements (Fe, Mn, Zn and Cu). Amongst these Fe and Zn was found to be highest (314.60ppm) and (36.10ppm). Heavy metals (Ni, Pb, Cr, Cd) concentration was analyzed by atomic absorption spectrophotometer (AAS), the Cr content was found to be highest 13.25%, followed by Ni 2.35%. Six metabolites identified through GC-MS analysis in methanolic extract of P. arrhizus. The major metabolites are Butanal, 3-methyl-, Benzene, methyl-, Benzene, 1,3-bis(1,1-dimethylethyl)-, and 1-Hexanol, 2-ethyl- covering a peak area of 3.34%, 2.87%, 2.24% and 1.99%.