Plants are rich source of the traditional medicine water hyacinths pose serious challenges to humanity and the environment. Consider the extent of the menace with the growth and spread of the plant and the difficulty in achieve a single, normally acceptable control method, it is attractive increasingly essential to explore the potentials of the plant. Phytochemical presence of Tannin, Saponin, Flavonoids, Steroids, Alkaloids and Polyphenol. GC-MS analysis of bioactive mixtures major Twelve bioactive compounds parts are distinguished as Retention time [8.95] Cyclohexasiloxane, dodecamethyl, biological activity of Emollient, in personal care products, lubricant and de-foaming agent. Hexadecanoic acid, methyl ester R.Time [15.97], Tetracosamethyl-cyclododecasiloxane R.Time [24.24], 9,12,15 Octadecatrienoic Acid R.Time [30.24]. In-vitro anti-oxidant activity of (A) DPPH scavenging activity of 12.5(μM) - Maximum value 1.42±0.71 and 100(μM) minimum value 19.76±1.27 IC50 (μM) ±SEM 35.13±1.79 (B) H2O2 scavenging activity of Maximum value 12.5(μM) 2.53±0.52 and 100(μM) minimum value 23.63±1.68 IC50 (μM) ±SEM 47.87±3.07 (C) Nitric oxide scavenging activity of Maximum value 12.5(μM) 3.82±0.64 and 100(μM) minimum value 24.41±2.16 IC50 (μM) ±SEM 39.31±4.87 (D) FRAP assay of maximum value of 12.5(μM) 2.58±0.58 and minimum value 100(μM) 20.41±1.83 IC50 (μM) ±SEM 38.85±3.85. In-vitro anti-oxidant activity of treated with high potential plant growth Eichhornia Crassipes.