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VOL. 6, ISSUE 5 (2021)
Evaluation of anti-arthrotic activity of Xanthoxylum oxyphyllum, an indigenous plant
Authors
Jayant Chatterjee, Lipika Buragohain, Chandana Choudhury Barua, Pritam Mohan, Manisha Dutta
Abstract
Xanthoxylum oxyphyllum plant was evaluated for anti-arthrotic properties and mRNA/protein expression of few molecular markers like IL-10, NF-kB and Nrf2 in rats and mice. The Taxonomist of Assam Agricultural University identified the plant as Zanthoxylum oxyphyllum Edgew. which is synonym of plant Xanthoxylum oxyphyllum and provided collection number as 5176. The calculated LD50 was found to be below 2000 mg/kg and 1000 mg/kg was taken as the safe dose for further evaluation according to OECD-423 guidelines. Anti-arthrotic property of the plant was evaluated by the means of Adjuvant Induced Arthritis (AIA) model. Group I served as negative control and Group II served as positive control and received oral Meloxicam suspension. Group III, IV & V were administered 10, 30 & 100 mg/kg of ethanolic extract per oral, respectively. Body weight and paw volume of both paws were measured on the Ist day and Arthritis was induced by injecting 0.1 ml of FCA (Freund’s Complete Adjuvant) into the left hind paw of all rats except negative control group. Paw volumes were measured to evaluate the therapeutic effect of the extract on primary lesions and inhibition of secondary lesions. Medium dose significantly increased body weight but could not inhibit primary lesions as efficiently as standard but inhibited secondary lesions by a great extent. The activation and upregulation of Nrf2 gene as confirmed by Reverse Transcriptase-PCR strengthened the antioxidant property. The ethanolic leaf extract of Xanthoxylum oxyphyllum, possesses Anti-arthrotic property as evidenced from the studies.
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Pages:1219-1225
How to cite this article:
Jayant Chatterjee, Lipika Buragohain, Chandana Choudhury Barua, Pritam Mohan, Manisha Dutta "Evaluation of anti-arthrotic activity of <em>Xanthoxylum oxyphyllum, </em>an indigenous plant ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 1219-1225
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