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VOL. 6, ISSUE 5 (2021)
In vitro anticancer activities and cytotoxic studies of the leaf extracts of Ichnocarpus frutescens (L.) WT. aiton against HeLa cell line
Authors
Rajanna L, Ashwini
Abstract
Ichnocarpus frutescens (L.) W.T. Aiton is an important medicinal plant of the family apocynaceae used to cure various ailments. Leaf extracts of I. frutescens was prepared by using different solvents like petroleum ether, chloroform, ethyl acetate, ethanol and water to screen their anticancerous activity against HeLa (Human cervical cancer cells) cell lines. Chloroform leaf extract showed significant cytotoxicity in MTT assay against HeLa cell line with IC50 value 188.6µg/ml. It was further tested for their apoptotic activity, caspase-3 expression and Cell Cycle analysis using AnnexinV-PI staining, caspase-3 antibody staining and Propidium Iodide Staining respectively. The results of the present study indicated that I. frutescens have anticancerous effect on the HeLa cancer cell line with the induction of apoptosis, caspase-3 enzyme expression and cell cycle arrest. This plant may be recommended for the formulation of new therapeutic drugs to cure human cervical cancer.
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Pages:1086-1091
How to cite this article:
Rajanna L, Ashwini "<em>In vitro</em> anticancer activities and cytotoxic studies of the leaf extracts of <em>Ichnocarpus frutescens </em>(L.) WT. aiton against HeLa cell line ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 1086-1091
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