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VOL. 6, ISSUE 5 (2021)
Phytochemical analysis of the ethanolic extract of Hypolepis glandulifera brownsey et chinnock using UV-VIS, FTIR and HPLC
Authors
R Amster Regin Lawrence, J John Peter Paul, C Iniya Udhaya, S Athi Lakshmi, P Anusathya Priya, E Fredrick Raja, S D K Shri Devi
Abstract
The aim of the present study was to screen the phytoconstituents composition of ethanol extract of Hypolepis glandulifera Brownsey et Chinnock collected from Kothiyar, located in Kanyakumari district, Tamil Nadu, India. The phytochemical screening of ethanol extract was estimated using the standard procedure for UV-Vis spectroscopic, HPLC and FTIR. The UV-Visible spectrum showed ten compounds separated at the wavelength of at the nm of 1024, 888, 664, 606, 532, 500, 450, 364, 312 and 236 with the absorption 0.247, 0.287, 0.886, 1.348, 1.456, 1.794, 2.268, 1.204, 1.755 and 1.650 respectively. The qualitative HPLC fingerprint profile displayed five compounds at different retention times. The profile displayed two prominent peaks at the retention time of 2.117min and 2.800min, followed by three moderate peaks were predicted at the retention time of 1.207min, 3.220min and 4.043min. The FTIR analysis recognized different peaks at 624.89, 670.22, 778.22, 1056.92, 1173.6, 1363.58, 1423.37, 1514.98, 1606.59, 1730.99, 2925.81, 3062.75 and 3381.95cm-1 with the presence of functional groups such as naphthalenes, aldehydes, 1,2,3-trisubst benzenes, alkyl sulfoxides, sulfonic acids, sulfonyl chlorides, carboxylic acids, triazine compounds, β-diketones, δ lactones, aliphatic compounds, aromatic and unsaturated compounds and alcohols and phenols.
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Pages:1379-1381
How to cite this article:
R Amster Regin Lawrence, J John Peter Paul, C Iniya Udhaya, S Athi Lakshmi, P Anusathya Priya, E Fredrick Raja, S D K Shri Devi "Phytochemical analysis of the ethanolic extract of <em>Hypolepis glandulifera </em>brownsey <em>et</em> chinnock using UV-VIS, FTIR and HPLC ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 1379-1381
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