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VOL. 6, ISSUE 5 (2021)
In vitro antioxidant and antihemolytic potential of methanolic flower extract of Crinum asiaticum
Authors
B S Ramesh
Abstract
Crinum asiaticum is a member belonging to the family Amaryllidaceae. Commonly called Spider lily, Fresh flowers were made into fine paste with methanol solvent using pestle and mortar. The fine paste of flower was soaked in respective solvent for 4–5 h in water bath at 50°C. After hot extraction, the extract was filtered through the Whatman No.1 filter paper and allowed to evaporate. The condensed extract was subjected to antioxidant free radical scavenging assay and antihemolytic activity. Determination of antioxidant activity of methanolic flower extract was performed using two assays such as 2, 2,-diphenyl-picrylhydrazyl (DPPH) and 2,2’-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) free radical scavenging assay using quercetin as standard. In both DPPH, ABTS radical scavenging assays, methanol extract showed remarkable antioxidant activity with an IC50 value of 600μg/ml. Hemolysis activity revealed positive result for crude flower extract tested for their antihemolytic potential. Triton X-100 and Phosphate buffer saline were used as positive and negative control respectively. From the result it could be concluded that C. asiaticum flower extract owned significant antioxidant and antihemolytic potential.
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Pages:1283-1287
How to cite this article:
B S Ramesh "<em>In vitro</em> antioxidant and antihemolytic potential of methanolic flower extract of <em>Crinum asiaticum</em> ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 1283-1287
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