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VOL. 6, ISSUE 5 (2021)
in vitro and in vivo antidepressant activity of Sarcostigma kleinii (wight & arn.) family-Icacinaceae
Authors
Elizabeth Abraham P, Jyoti Harindran
Abstract
Depression, or major depressive disorder, is a mental health condition marked by a feeling of sadness, isolation and despair that affects how a person thinks, feels and functions. Lack of sufficient scientific evidence indicating the utility of this plant in the treatment of major depressive disorder prompted us to investigate activity of the plant. In in vitro activity neuroprotective effect of ethyl alcoholic extract at concentrations 6.25, 12.5, 25, 50 and 100 μg/ml on IMR32 (Neuroblastoma cells) were determined by MTT assay and Reactive Oxygen Species measurement using flourascence analysis. In In-vivo pharmacological screening method anti-depressant activity of extract at doses of 200mg/kg and 400 mg/kg was assessed by Sucrose preference test, Locomotor activity test, Forced swim test, and Tail suspension test using female Swiss Albino mice. The results of in vitro screening showed that percentage of cell viability significantly increased when treated with a concentration of 12.5 μg/ml extract when compared with other treated concentrations. The decrease in ROS generation in cells showed that it can be neuroprotective due to increased cell viability. In-vivo pharmacological screening data supported antidepressant potential of extract of Sarcostigma kleinii by these models of depression. The present study exhibits anti-depressant potential of Ethyl alcoholic extract of Sarcostigma kleinii (EAESk) in depression like symptoms in mice exposed to unpredictable chronic mild stress.
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Pages:1394-1398
How to cite this article:
Elizabeth Abraham P, Jyoti Harindran "<em>in vitro </em>and <em>in vivo</em> antidepressant activity of <em>Sarcostigma kleinii </em>(wight <em>& </em>arn.) family-<em>Icacinaceae</em> ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 1394-1398
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