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VOL. 6, ISSUE 5 (2021)
An influence of coco peat and vermicompost on successful Ex vitro rooting of micropropagated plantlets of Oxystelma esculentum R. Br
Authors
R Sabitha, T Francis Xavier, S Balavivekananthan, A Freeda Rose
Abstract
In the present study a simple and efficient in vitro propagation system was developed from nodal explants of Oxystelma esculentum R. Br by using different plant growth regulators. The nodal explants inoculated on the 6-benzyladenine phosphate and Kinetin individually and with combinations for an induction of shoots. The MS medium supplemented with 1.0mg/L of BAP with 1.0 mg/L of Kinetin combinations inducing (95%) the shoots effectively with the shoot length of 7.7±2.2. The well-developed shoots were cut and transferred to IAA (Indole-3-acetic acid) & IBA (Indole-3-butyric acid) individually and with combinations of half strength MS medium for root induction. The high percentage (95%) of rooting was observed in 0.5 mg/L IBA concentration in the mean number of 15.8±1.4 with 12.10±3.3 root length. Simultaneously the well elongated shoots were treating with rooting hormones with various concentrations for 5 minutes and transferred to hardening with the use of coco peat and vermicompost combined with soil with different ratios. The high mean numbers of roots were observed on the combinations of NAA and IBA (200 mg each) concentrations then the 100% survival rate was observed on soil:cocopeat: vermicompost (2:1:1) ratios after 8 weeks of hardening.
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Pages:1520-1526
How to cite this article:
R Sabitha, T Francis Xavier, S Balavivekananthan, A Freeda Rose "An influence of coco peat and vermicompost on successful <em>Ex vitro</em> rooting of micropropagated plantlets of <em>Oxystelma esculentum</em> R. Br ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 1520-1526
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