Medicinal plants is the source for the search of many novel therapeutic compounds in developing countries. Before used as medicine, drugs from plant origin must be ensured as safe. The present work focused to study the in vivo toxicity effects of the ethanol extracts of Eleusine coracana varieties (powder of C014, C015 and flake of C014, C015) and its formulation (1:1 ratio of C014, C015 powder and 1:1 of ratio C014, C015 flake) were carried out in vivo on albino rats. Seven groups of albino rats were treated with five different doses of the ethanolic extracts of Eleusine coracana varieties (powder of C014, C015 and flake of C014, C015) and its formulation (1:1 ratio of C014, C015 powder and 1:1 of ratio C014, C015 flake) orally for 14 days. General appearance and behavior were observed for 14 consecutive days. Acute toxicity studies results showed that up to the tested dose of 2000 mg/kg bwt in extract treatments, throughout the 14 days of treatment, the extract does not produced any toxicity symptoms. No toxicity related symptoms were observed in doses of finger millet powder and formulation extracts treated rats groups. So the LD50 values of the tested finger millet extracts were more than 2000 mg/kg bwt.