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VOL. 6, ISSUE 5 (2021)
Molecular characterization through ITS sequencing of the native isolates of Trichoderma species
Authors
Tulipa De, Ayon Roy
Abstract
The genus Trichoderma are used in biological control programs to control phytopathogens. The secondary metabolites produced by Trichoderma have antifungal as well as mycoparasitism actions, defense response induction and resistance in host plants which act synergistically. Different molecular biology techniques used for molecular characterization of the Trichoderma genus include UP-PCR (Universal Primer-Polymerase Chain Reaction) and RAPD (Random Amplification of Polymorphic DNA). In the present investigations, molecular characterization of the native isolates of Trichoderma species was done using ITS sequencing. Two best strains of Trichoderma i.e. UBT-18 and T21 were used and molecular characterization was done using ITS1 and ITS4 Primers. Distance trees of nucleotide sequence of UBT 18 using ITS1 and ITS 4 primers indicated that the test isolates had belong to Trichoderma harzianum whereas distance trees of nucleotide sequence of T 21 using ITS 1 and ITS4 primers primers indicated that the test isolates had belong to Trichoderma asperellum.
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Pages:681-684
How to cite this article:
Tulipa De, Ayon Roy "Molecular characterization through ITS sequencing of the native isolates of <em>Trichoderma </em>species ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 681-684
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