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VOL. 6, ISSUE 5 (2021)
DNA barcoding and chemical profiling in medicinal and aromatic grass species, wild Cymbopogon citratus (DC) Stapf
Authors
Sree Latha K, Hemalatha J, Vinutha M, Thara Saraswathi K J
Abstract
Cymbopogon species are high value aromatic grasses accounting for about 140 species worldwide. Globally renowned Cymbopogon citratus (C.citratus) which is native to South West Asia is occasionally found growing in wild locations of Karnataka. Natural habitat of Cymbopogon species, phenotypically appearing as C. citratus was sourced out at Thonnur Lake, Mandya district, Karnataka. Fresh leaves were subjected to hydro distillation for essential oil extraction. Qualitative analysis of the essential oil was made by GC-MS analysis. Major constituents in the essential oil were found to be geranial (48%), neral (34%) and beta-myrcene (6.16%). These are the biochemical marker constituents of C.citratus. Besides, geranial and neral constituent composition in total was 82% which was found to be higher when compared to the standard references on essential oil composition reported in the cultigen relative, RRL-J (72-75%) developed from Central Institute of Medicinal and Aromatic Plants, Lucknow. In addition, molecular marker based taxonomical identification of the wild Cymbopogon species was performed using DNA barcoding technique. Standard DNA barcode primer ITS-2 was used in the study. Morphological observation, cytogenetic, biochemical and molecular analyses validated the wild Cymbopogon species as C.citratus. Uninhabited aromatic grasses are innately adapted to withstand environmental stress which results in the production of higher amounts of secondary metabolites and diverse components naturally and occasionally, adapt newer pathways and mechanisms to produce novel compounds for sustenance. This presents an opportunity to explore natural compounds for the benefit of mankind. The present study suggests utilization of digitally authenticated native gene pool of C.citratus in molecular breeding program and genetic resource management strategies.
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Pages:736-741
How to cite this article:
Sree Latha K, Hemalatha J, Vinutha M, Thara Saraswathi K J "DNA barcoding and chemical profiling in medicinal and aromatic grass species, wild <em>Cymbopogon citratus </em>(DC) Stapf ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 736-741
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