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VOL. 6, ISSUE 5 (2021)
Optimization of chitinase synthesis or chito-oligosaccharide production using antibiotic sensitive Cellulosimicrobium cellulans isolated from mushroom compost
Authors
M S Girase, A E Gawade, S N Patil, V B Gaikwad
Abstract
Chito-oligosaccharides are having increasing applications in fields like food, cosmeceutical, waste management, agriculture and medicine. Chito-oligosaccharides play important role in plant defense. There is a great demand for high production of chito-oligosaccharide with superior properties. Physical and chemical methods of chito-oligosaccharide production demands high energy inputs or they are not environment friendly. For this better option is enzymatic production. In the present work, attempts are made for enzymatic production of chito-oligosaccharide using chitinase producing culture. For achieving this, screening, isolation of chitinase producer (chito-oligosaccharide yielding) organism, optimization of fermentation conditions for chitinase production and finding optimum conditions for chitinase activity was done. Chitinase producing Cellulosimicrobium cellulans strain IARI-ABL-30 was isolated from mushroom compost. Organism was sensitive to commonly used antibiotics hence can be safe organism for production of chito-oligosaccharides from chitin. Meat extract, 45oC, pH 7 and incubation period of 4 days are optimum conditions for chitinase mediated chito-oligosaccharide production from biological chitin by Cellulosimicrobium cellulans strain IARI-ABL-30.
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Pages:721-728
How to cite this article:
M S Girase, A E Gawade, S N Patil, V B Gaikwad "Optimization of chitinase synthesis or chito-oligosaccharide production using antibiotic sensitive <em>Cellulosimicrobium cellulans</em> isolated from mushroom compost ". International Journal of Botany Studies, Vol 6, Issue 5, 2021, Pages 721-728
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