Indian mustard is considered as nutritionally rich oilseed crop. The aim of current research was to study, the phytochemical and nutritional potential of selected cultivars with an intent to provide therapeutic benefits to the human. The phytochemical present in plant parts (seeds) were extracted by overnight dipping with methanol, ethanol, acetone, distilled water. Three types of mustard seed were taken for analyses that are Brassica juncea, Brassica nigra, Sinapis alba. Qualitative phytochemical analysis was done for various phyto-constituents like alkaloids, tannins, flavonoids, steroids, cardiac glycosides and saponins. The result of analysis shows phytochemical constituents of different mustard seeds have therapeutic effect which will be helpful in studying the future pharmacology.